3D imaging of large samples in light-sheet microscopy (Ref. UC3M OT_166)

Commercial systems for 2D imaging are rapid and reliable, but unsuitable for 3D imaging. This technology allows high-quality 3D images to be generated by means of a light sheet eliminating physical movement from the sample and increasing the acquisition rate and the total field of view as a result of a lenslet array combined with mirror galvanometers. The invention is useful for microscopy, particularly for images of complex biological samples.

INNOVATIVE ASPECTS: 

·     3D imaging by means of a light-sheet microscope

·     Combination of elements in an innovative configuration:

·     Lenslet array.

·     Mirror galvanometers.

·     Spatial light modulator.

·     Stationary positioning of the sample when a 3D image is obtained

·     Imaging of complex biological samples, including transparent or semi-transparent samples

COMPETITIVE ADVANTAGES:

·     The sample does not need to be moved for 3D imaging.

·     A lenslet array is used instead of the conventional microscope objective lens.

·     3D scanning by means of the combination of a lenslet array and mirror galvanometers.

·     Mirror galvanometers allow the selection of the element to be sampled, dispensing with the need to physically move the sample.

·     An aberration corrector (e.g. spatial light modulator) can be included.

·     High speed for taking a 3D image.

STAGE OF DEVELOPMENT: Available for demonstration.

INTELLECTUAL PROPERTY RIGHTS: Patent applied.